Nanoparticle analysis

A new methode of Nanoparticle measurement allows the individual visualisation and size determination of nanoparticles in suspension. 

- Size range: 10 to 1000 nm

- Usable for all types of nanoparticle material

- Broad sprectrum of solvants

- Minimal sample volume needed (300 Microliter)

- Polydisperse samples are well measured

- Long term measurements up to 12 hours

- Material analysis by measuring the intensity of ligh scattering

- Concentration determination of nanoparticles and virus

- Measurement of fluorescently labelled particles

NEW: Zeta-Potential measurement

Zeta Potential Nanoparticle Tracking Analysis (Z-NTA) adds measurement of surface charge to simultaneous reporting of size, light scattering intensity, fluorescence and count, and does so particle-by-particle. 

No other methodology comes close to providing such simultaneous, multiparameter nanoparticle characterisation.

· Zeta potential is measured particle-by-particle, simultaneously for the population.

· Simultaneously each particle also has size and light scattering intensity reported.

· Polydisperse and complex suspensions are readily characterised.

· No labeling is required however a fluorescence mode further differentiates suitable-labeled sub-populations.

· This data set is validated by real time observation of particles moving under both electrophoresis and Brownian motion.

As a consequence:

· Variation in zeta potential with size is analysed.

· Positively and negatively charged particles are reported separately, should they exist together.

· Sub-populations of similar sized particles of different materials, differentiated by their propensity to scatter light, are separately reported and counted.

· Changes in Zeta Potential distribution with pH, concentration and temperature can be studied.

· Aggregation and flocculation can be studied quantitatively in real-time.

· Suitable labeling can highlight one sub-population for analysis despite high background noise.

For further information on Zeta-Potential measurement please see the Application Note.

Independent comparison of NanoSight NTA versus DLS:

Publication University Leiden: Comparison NanoSight versus DLS

- Link to summary

- Link to article 

New instrument: NanoSight NS500

- Fluorescence

- Fluid handling with auto sample preparation and in-situ cleaning

- Temperature control 15° - 55°C

- Zeta potential (as option)

Recorded WEBINARS are available on the NanoSight website.

Subjects are under others Protein Aggregation or Exosome Characterization.

Available models:

NanoSight LM20

NanoSight LM10

NanoSight LM10-HS (with highly sensitive EMCCD camera)

NanoSight NS500

NanoSight NS200

Application

Size determination of nanoparticles and virus in the range of 10 bis 1000nm in suspension.

Concentration determination of virus and particles

- Nanotoxicology 

- Vaccine production (Virus, Phage, Macrophages)

- Particle alysis in serum

- Exosomes, ectosomes, microvesicles, ectocytosis

- Wear debris tests in implants

- Fuel additives

- Toner

- Metals

- Metal oxides

- Fluorescence, Quantumdots, Q-dots

- Zeta potential of nanoparticles

- etc.